Acquired brain injury (ABI) refers to a broad range of mechanisms of brain injury, including trauma, stroke, hypoxia and tumour. The effects of ABI on functioning and subsequent recovery are highly variable across individuals and depend on the mechanism and severity of injury. Magnetic Resonance Imaging (MRI) provides a powerful tool for characterizing effects of injury on the underlying neurophysiology. In this workshop, we will provide an overview of the historic role of neuroimaging and MRI in evaluating ABI. We will then examine advanced MRI techniques which have been used to obtain greater insight into brain injury. Topics will include: diffusion-weighted imaging techniques, which may be used to interrogate changes in tissue microstructure; blood oxygenation level dependent functional MRI which has been used to characterize brain function both at rest and during cognitive tasks; along with techniques to quantify cerebrovascular function and cerebral blood flow. At the end of this workshop, participants will have a greater understanding of the current state of knowledge in neuroimaging of ABI.